How does hydrogen peroxide affect catalase

Northrop, J. Williams, J. Google Scholar. Adair, G. Ferry, R. Keilin, D. Download references. You can also search for this author in PubMed Google Scholar. Reprints and Permissions. Reaction Between Catalase and Hydrogen Peroxide. Nature , 41—43 Download citation. Issue Date : 12 July Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Lee Woodcock.

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But since hydrogen peroxide tends to decompose slowly by itself, looking for extra bubbles was a challenging endeavour. This relied on the chemistry of phenolphthalein, well-known today to students as an acid-base indicator.

Phenolphthalein is colourless in acid but turns a deep pink in a basic solution. In this case, though, the important feature is that phenolphthalein can be reduced with zinc into colourless phenolphthalin, which along with a base is present in the test reagent.

In the usual process, a drop of alcohol is added to an unknown stain to dissolve any hemoglobin that may be present, followed by rubbing with a swab that has been treated with the Kastle-Meyer reagent. A drop of hydrogen peroxide is then applied to the swab. If hemoglobin is present, the hydrogen peroxide decomposes to yield oxygen that in turn oxidizes the phenolphtalin to phenolphthalein. Since the solution is basic, a pink colour develops indicating the presence of blood.

The test is very sensitive, but is not specific for human blood. Animal blood will also yield a positive reaction as will oxidizing agents such as some metal ions. The technique is to spray the suspect area with a solution of luminol and hydrogen peroxide.

If blood is present, the peroxide will yield oxygen that then reacts with luminol to produce a blue glow. This reaction was first noted in by the German chemist H. Albrecht and was put into forensic practice in by forensic scientist Walter Specht. Even dried and decomposed blood gives a positive reaction with the blue glow lasting for about 30 seconds per application.